Section/Topic

Item No

Check List Item

Title and Abstract

1a

Identification as In-vitro study in the title

1b

Structured abstract of Aim, Materials and methods, Results & Conclusion

Introduction

Background

2a

Specific background leading to a clear rationale

Objectives

2b

Specific Hypothesis conforming to the title and other objectives (if any)

Methods

Samples/Specimens

3a

Selection criteria for samples

3b

Description of samples if obtained from same specimen (like multiple samples obtained from single tooth in micro-tensile bond testing)

3c

Sample preparation prior to allocation to groups

Study design

4a

Elaborate experimental setup / design

4b

Number of groups and allocation ratio

Interventions

5

Detailed description on composition, strength (s)/ percentage / concentration of the intervention and frequency and dosage of administration

Outcomes

6a

How was the primary and secondary outcome measured

6b

Technical details on the use of appropriate quantifying methods used to measure outcome

6c

If any other outcome that was measured after the completion of the study, with reasons

Sample size

7a

How was sample size determined

7b

What was the meaningful difference or precision for the study

7c

If applicable, rationale for number of samples required for sub-group analysis

Randomisation

Sequence generation

8a

Method used to generate random sequence

8b

Type of randomisation

Allocation concealment

9

Mechanism used to implement random allocation sequence (numbered envelops) of the groups

Implementation

10

Who generated the randomisation sequence, sample collection and allocation to groups

Blinding

11a

If done, who were blinded to the study (Investigator, operator and outcome assessor)

11b

If applicable, description of similarity of the material/agent/technique tested

Statistical Methods

12

Statistical methods used to compare groups for primary and secondary outcomes

Results

Samples

13

Samples lost due to handling process not available for analysis

Baseline data

14

If applicable, a table showing the morphological characteristics and dimensions for each group

Outcomes and estimation

15a

For each primary and secondary outcome, results for each group, and the estimated effect size and its precision (such as 95% CI or SE whichever applicable)

15b

For binary outcomes, presentation of both absolute and relative effect sizes is recommended

Ancillary analyses

16

Results of any other analyses performed

Discussion

Interpretation

17

Interpretation consistent with results, balancing merits and demerits and considering other relevant evidence, with focus on primary objective

Applicability

18

Meaningful translation to relevant information applicable in understanding material behaviour/ clinical technique

Limitations

19a

Study limitations

19b

Addressing potential for bias

Conclusion

20

Is the conclusion based on the findings of the study

Funding

21

Sources of funding and other support, role of sponsors

Conflict of interest

22

Disclosure of potential conflict of interest

Study registration

23

Protocol registration number

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